Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation.

The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily glucose and xylose. However, strains of Saccharomyces cerevisiae presently used in bioethanol production ferment glucose but not xylose. Yeasts engineered to ferment xylose do so slowly, and cannot utilize xylose until glucose is completely consumed. To overcome these bo...

Engineered production of fungal anticancer cyclooligomer depsipeptides in Saccharomyces cerevisiae.

Two fungal cyclooligomer depsipeptide synthetases(CODSs), BbBEAS (352 kDa) and BbBSLS (348 kDa) from Beauveria bassiana ATCC7159, were reconstituted in Saccharomyces cerevisiae BJ5464-NpgA, leading to the production of the corresponding anticancer natural products, beauvericins and bassianolide, respectively. The titers of beauvericins (33.8 ± 1.4 mg/l) and bassianolide (21.7± 0.1 mg/l) in the ...

Optimization of butanol pathway in metabolically engineered Saccharomyces cerevisiae

Engineered Saccharomyces cerevisiae that produces 1,3-propanediol from D-glucose.

AIMS Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost. METHODS AND RESULTS By using D-glucose as...

Biosynthesis of Zinc Oxide Nanoparticles using Intracellular Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities

Introduction: Attention to the biosynthesis of nanoparticles (NPs) has been increased recently since they are cost-effective, eco-friendly, and potential alternatives to chemical and physical methods. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using an intracellular extract of Saccharomyces cerevisiae. Moreover, it was attempted to evaluate their antibacterial and antioxi...